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研究未讨论温度对探针回收率的影响。（2）流速可直接影响待测物质的回收率，探针回收率可随流速增大而减小。体内微透析实验要求在一定的时间内获得足够定量测量的样品容积，经对不同流速（0.5,1,2,3,4 μL/min）体外回收率的考察，流速在2 μL/min时符合上述要185 求，且增量法和减量法测得的探针回收率相一致，由此证明体内反透析法（减量法）测得的探针回收率可用于体内透析（增量法）物质浓度的计算。 通过对微透析探针回收率的研究，确定了反透析法可作为钩藤碱体内回收率的测定方法，回收率的测定是计算体内药物浓度的基础。实验结果还显示不同探针间回收率存在一定的差别，因此，每次探针植入后都应测定回收率用于本次体内药物浓度的计算。本实验还对190 灌流速度、采样量及采样时间等微透析参数进行了初步的研究，最终选择流速为2 μL/min，采样时间为15 min，样品量为30 μL作为体内微透析参数设置的参考值。 5.结论 微透析技术可用于钩藤碱的体内药动学研究，反透析法可作为体内研究中钩藤碱探针回收率的测定方法。 195 [参考文献] (References) [1] E. Guihen, W.T. O Connor, Current separation and detection methods in microdialysis the drive towards sensitivity and speed, Electrophoresis. 30 (2009) 2062-2075. [2] Ungerstedt U, Hallstrom A. In vivo microdialysis- a new approach to the analysis of neurotansmitters in the brain [J]. Life Science, 1987, 41 (7) :861 - 864. 200 [3] Pradyot Nandi, Susan M. Lunte Recent trends in microdialysis sampling integrated with conventional and microanalytical systems for monitoring biological events: A review[J] . Analytica Chimica Acta , 2009，651:1-14. [4] Bosche B, Dohmen C, Graf R, et al. Extracellular concentrations of non-transmitter amino acids in peri -infarct tissue of patients predict ma lignant middle cerebral artery infarction[J]. Stroke, 2003, 4(12): 2908-2913. [5] Zhou, S. Zhou. Antihypertensive and neuroprotective activities of rhynchophylline: The role of 205 rhynchophylline in neurotransmission and ion channel activity[J]. J. Ethnopharmacol. 132 (2010)15-27. [6] Elizabeth C.M, de Lange, A.G. de Boer, et al. Methodological issues in microdialysis sampling for pharmacokinetic studies[J]. Advanced Drug Delivery Reviews, 2000, 45:125-148. [7] Christoffer Bundgaard , Martin Jørgensen , Arne Mørk. An integrated microdialysis rat model for multiple pharmacokinetic/pharmacodynamic investigations of serotonergic agents[J].Journal of Pharmacological and 210 Toxicological Methods, 2007, 55(2): 214-223. [8] Nele Plock, Charlotte Kloft. Microdialysis-theoretical background and recent implementation in applied life-sciences[J]. European Journal of Pharmaceutical Sciences, 2005, 25:1-24. [9] Yannick Tholance, Gleicy Barcelos, Isabelle Quadrio,et al. Analytical validation of microdialysis analyzer for monitoring glucose, lactate and pyruvate in cerebral microdialysates[J]. Clinica Chimica Acta, 2011, 412:647-654. 215 [10] Stenken J A．Methods and issues in microdialysis calibration[J]．Anal Chimica Acta，1999, 379：337. 学术论文网Tag：代写论文 代写职称论文 论文发表 代写医学论文 |
