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采用激光粒度仪测定不同温度下自组装胶束的粒径，实验结果表明叶酸修饰的PNiPAAm-co-PCL自组装载药胶束具有温度敏感特性。 粒径随温度变化的趋势图2503003504004505005503535.836.637.438.23939.840.641.4温度（°C）粒径（d.nm） 图12 叶酸修饰的两亲性PNiPAAm-co-PCL紫杉醇纳米胶束的温度敏感性实验 3结论 160 本实验以叶酸修饰的两亲性PNiPAAm-co-PCL作为载体材料，采用高分子自组装的方式包载药物和超顺磁微粒，制备叶酸修饰的两亲性PNiPAAm-co-PCL紫杉醇纳米胶束，对影响紫杉醇纳米胶束制备的处方因素和工艺条件进行考察和优化, 制备了一种具有温度敏感性的叶酸修饰PNiPAAm-co-PCL紫杉醇自组装纳米胶束。由透射电镜和粒度分布结果可知，当载体材料叶酸修饰的PNiPAAm-co-PCL与紫杉醇投药量比为10：1，混合溶剂选取165 CH2CL2和丙酮，体积比为2：1，选取2%PVA作为乳化剂，37%超声5min ，所制得的纳米胶束最理想，纳米胶束大多数呈很规整的球型平均直径为179.2nm，粒径分布窄。 [参考文献] (References) [1] Rapoport N. Physical stimuli-responsive polymeric micelles for anti-cancer drug delivery[J]. Progress in 170 Polymer Science. 2007:32(8-9):962-990. [2] Yang T, Cui FD, Choi MK, et a1． Liposome formulation of paclitaxel with enhanced solubility and stability[J]．Drug Deliv, 2007, l4(5):301-8. [3] 张永, 涂家生, 沈雁, 等．注射用紫杉醇聚合物小鼠体内药化动力学[J],中国药科大学学报, 2007, 38(4):343-346. 175 [4] Harada A, Kataoka K. Supramolecular assemblies of block copolymers in aqueous media as nanocontainers relevant to biological applications[J]. Progress in Polymer Science. 2006, 31(11):949-982. [5] Ren J, Hong HY, Ren TB, et al. Preparation and characterization of magnetic PLA-PEG composite particles[J]. Materials Letters. 2005, 59(21):2655-2658. [6] Zhang JX, Qiu LY, Jin Y, et al. Controlled nanoparticles formation by self-assembly of novel amphiphilic 180 polyphosphazenes with poly(N-isopropylacrylamide) and ethyl glycinate as side groups[J]. Reactive & Functional Polymers. 2006, 66(12):1630-1640. [7] Allen TM , Cullis PR．Drug delivery system: entering the mainstream[J], Science, 2004, 303(5665):1818-22. 学术论文网Tag：代写论文 代写职称论文 论文发表 代写医学论文 |
